UNIVERSITY
OF NIGERIA, NSUKKA REG.
NO: ……………………………………………………………
FACULTY OF
PHARMACUTICAL SCIENCES
DEPARTMENT
OF PHARMACUETICS
SERIAL NO: ………………………………………………..
2012/2013
SESSION RESIT PROFESSIONAL EXAMINATION
PHM225: PRATICAL
BASIC PHARMACEUTICAL MICROBIOLOGY
FEBRUARY 22,
2014
SECTION A
INTRUCTIONS: 1. ANSEWR ALL QUESTIONS
2. DO NOT LEAVE
THE EXAMINATION HALL WITH THE QUESTION PAPER
TIME
ALLOWED: 3 HOURS
1.
(a) In pharmaceutical microbiology practical
examination, five organisms (F-J) were subjected to the following staining
methods namely Gram stain, spore stain, and Capsule stain. The results below
were obtained for each organism. From the list of bacteria below, suggest one
suitable name for each organism based on the result obtains for each respectively.
Organism
|
Gram
stain
|
Spore
stain
|
Capsule
stain
|
F
|
Negative
|
Spore absent
|
Capsule present
|
G
|
Positive
|
Spore present
|
Capsule present
|
H
|
Positive
|
Spore absent
|
Capsule absent
|
I
|
Negative
|
Spore absent
|
capsule absent
|
J
|
Positive
|
Spore absent
|
Capsule absent
|
Streptococcus pneumoniae,
Klebsiella pneumoniae, Bacillus subtills, Bacillus anthracis, E. coli
Staphylococcus aureus, Clostridium botulinum, Candida albicans, Mycplasma
pneumoniae.
(b) During simple staining exercise, the bacteria listed below were
stained and observe with Bright field microscope. Describe completely in one
sentence the morphology of each of them (i) Streptococcus
mutans (ii) Bacillus subtilis (iii)
Sarcina lutea (vi) Staphyloccoccus albus (v) Diplococcus pneumonia.
(c) State factors that could account for Bacillus sbtilis appearing
like Salmonella typhi during Gram stain.
2.
(a) In a practical class iml of brand of cough
syrup was diluted using tenfold serial dilution method . Replicate drops were
plated from tested %. The following count were obtained after 24hrs incubation
at 37 degree: 33, 33, 34, 31 and 35. Estimate the bioload of this syrup, in
cfu/ml. volume of drop = 0.015ml.
(b) With one correct sentence state one specific use of the
following in a Pharmaceutical microbiology laboratory: (a) centrimide agar (b)
MacConkey agar (c) mannitol salt agar (d) selentie F Broth (e) simmon citrate
agar (f) autoclave (g) pour plate technique (h) hot air oven (i) petri dish(j)
Hot air oven.
UNIVERSITY
OF NIGERIA, NSUKKA REG.
NO: ……………………………………………………………
FACULTY OF
PHARMACUTICAL SCIENCES
DEPARTMENT
OF PHARMACUETICS
SERIAL NO: ………………………………………………..
2012/2013
SESSION RESIT PROFESSIONAL EXAMINATION
PHM225:
PRATICAL BASIC PHARMACEUTICAL MICROBIOLOGY
April 25,
2015
INTRUCTIONS: ANSEWR ALL QUESTIONS
TIME ALLOWED: 2 HOURS
1.
In one correct sentence mention one
specific use of the following media in
pharmaceutical microbiology laboratory namely (a) Cary-Blair medium (f)
Cetrimide agar (g) Nannitol salt agar (h) Selenite F Broth (i) Simmon citrate
agar (j) Xylose-lysine-Deoxycholate.7
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